PreCursor-M+

Qualitative detection of promotor hypermethylation of 2 cervical cancer associated genes (FAM19A4 and hsa-miR124-2).

Identify HPV positive women with high short-term progression to (pre)cancer.

Special Features:

  • Sample types:
    • Bisulfite-converted DNA isolated from physician collected cervical specimens or self-collected vaginal specimens
  • Clinical performance - Long-term data:   
    • Most evaluated and validated methylation assay to identify HPV positive women with progressing cervical disease in direct need for colposcopy or other follow-up procedures1,2
    • Demonstrating very high sensitivity for cervical cancer detection (95.0-100%) in HPV-positive cervical specimens2,3,4
    • Longitudinal data: The long-term risk for cancer with a negative PreCursor-M+ is lower than with a negative cytology result; for CIN3 the long-term risk is similar as cytology5,6 
  • Flexible:
    • Suitable for various sample types, including self-samples and common Liquid Based Cytology media types
    • Compatible with standard DNA extraction methods
  • Reliable:     
    • Build-in check for sample quality ensuring reliable results 

Precursor-M+ is a registered trademark of the legal manufacturer, Self-screen B.V., the Netherlands, and is distributed by Fujirebio Europe.

CE marked
PreCursor-M+

Product number 81352

72 Tests
Please contact your local Fujirebio representative for the availability of this product in your country.
Self-screen Precursor-M+

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  • Details

    Technical Specifications:

    • Method:
      • Multiplex real-time methylation-specific PCR assay
    • Target:
      • Promotor hypermethylation of 2 cervical cancer associated genes (FAM19A4 and hsa-miR124-2)
    • Sample types:
      • Cervical specimens stored in PreservCyt Solution or HC2 DNA Collection device
      • Self-collected vaginal brush/broom samples
    • Sample volume:
      • Input in the PreCursor-M+ reaction is 2.5 μL of bisulfite-converted DNA
    • Reaction time:
      • Total time to result: 4 - 24hrs. (incl. DNA extraction and bisulfite conversion; semi-automated or manual workflow)
        PreCursor-M+ assay:
      • Run time in real-time PCR cycler: within 90 minutes
      • Hands-on time: 20 minutes
    • Equipment:​​​​​​
      • Mic qPCR cycler (Bio Molecular Systems)
      • QPCR software (Bio Molecular Systems)
      • PreCursor-M+ v.1.0.mic template

     

    References:

    1. Kremer WW, Steenbergen RDM, Heideman DAM, Kenter GG, Meijer CJLM. The use of host cell DNA methylation analysis in the detection and management of women with advanced cervical intraepithelial neoplasia: a review. BJOG 2021;128:504-514
    2. Bonde J. et al. Int. J. Cancer. 2021;148:396-405. Methylation markers FAM19A4 and miR124-2 as triage strategy for primary human papillomavirus screen positive women: A large European multicenter study.

    3. Vink F.J. et al. Int J Cancer. 2020;147:1215-1221

    4. Hampl M. et al. Int J Cancer. 2022 ;1-8

    5. De Strooper et al. Cervical cancer risk in HPV-positive women after a negative FAM19A4/mir124-2 methylation test: A post hoc analysis in the POBASCAM trial with 14-year follow-up. Int. J. Cancer 2018;143(6):1541-1548 

    6. Dick et al. Long-term CIN3+ risk of HPV positive women after triage with FAM19A4/miR124-2 methylation analysis. Gynecol Oncol. 2019;154(2):368-373

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