PreCursor-M AnoGYN

PreCursor-M AnoGYN is a methylation-specific molecular assay to detect promoter hypermethylation of the human genes ASCL1 and ZNF582  in bisulfite-converted DNA prepared from human samples, for instance formalin-fixed paraffin embedded anal biopsies or anal swab specimens.

Special Features:

  • One-step PCR:
    • Multiplex format enabling simultaneous amplification and detection of the two targets and controls in a single reaction
  • Ready-to-use mastermix:
    • Just add bisulfite-converted DNA
  • Reliable:
    • Robust
    • Good reproducibility
    • Built-in check for sample quality and normalization.
For research use only
PreCursor-M AnoGYN

Product number 81524

72 Tests
Please contact your local Fujirebio representative for the availability of this product in your country.

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  • Details

    • Method: Multiplex real-time methylation-specific PCR assay
    • Target: Human genes ASCL1 and ZNF582
    • Built-in reference and sample quality control: β-actin (ACTB)
    • Calibrator: Contains a standard concentration of low-copy DNA fragments of the two targets ASCL1, ZNF582 and the control gene ACTB – also used for run validation
    • Sample types: Bisulfite-converted DNA prepared from human samples, for instance formalin-fixed paraffin embedded anal biopsies or anal swab specimens
    • Sample volume: 5 μL of bisulfite-converted DNA
    • Total time to result: Ranging between 2 to 24 hours depending on the level of automation (incl. DNA extraction and bisulfite conversion)
    • Equipment: Mic qPCR cycler (Bio Molecular Systems), Rotor-Gene Q 5plex system (Qiagen) or ViiA 7 qPCR system (Thermo Fisher Scientific)

     

    References:

    1. Costello, J.F., and Plass C. (2001) Methylation matters. J. Med. Genet. 38, 285-303.
    2. van der Zee R.P. , et al. Host cell deoxyribonucleic acid methylation markers for the detection of high-grade anal intraepithelial neoplasia and anal cancer. Clin. Infect. Dis., 68 (7); 1110-1117, 2019
    3. van der Zee R.P. , et al. Cancer risk stratification of anal intraepithelial neoplasia in human immunodeficiency virus-positive men by validated methylation markers associated with progression to cancer. Clin. Infect. Dis., 72 (12); 2154-2163, 2021
    4. van der Zee R.P. , et al. DNA methylation markers have universal prognostic value for anal cancer risk in HIV-negative and HIV-positive individuals. Mol. Oncol., 15 (11); 3024-3036, 2021, 2021
    5. Rozemeijer K, et al. Analytical validation and diagnostic performance of the ASCL1/ZNF582 methylation test for detection of high-grade anal intraepithelial neoplasia and anal cancer. Tumour Virus Res. 30;17:200275, 2023
    6. Steenbergen, R.D.M. et al. (2014) Clinical implications of (epi)genetic changes in HPV-induced precancerous lesions. Nat. Rev. Cancer 14, 395–405.

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